[Automated-sequencing] quality scores using phred from MegaBACE esd files

Sven Klages via autoseq%40net.bio.net (by klages from molgen.mpg.de)
Tue Nov 16 09:27:52 EST 2010

Hi Good Evening ;-)

> I can manage and use this .esd files to see sequence and make consensus
> analysis, but we need get really phred score in all fragments sequences.

Why don't you use 'phred' to re-basecall the ESD files to finally have 
SCF and/or PHD or whatever files?

CodonCode Aligner can handle these file types.


> Thnks a lot for any help
> regards
> Lic. Alex González
> Argentina GemaBiotech
> El 22/10/2009 13:09, Sven Klages escribió:
>> hat I'm new to these packages and therefore apologize if i'm
>> submitting an obvious question.
>> I have received a set off .SFF files that I would like to process/align.
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  Sven  Klages
  Max Planck Institute for Molecular Genetics
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