[Automated-sequencing] Re: Need help with converting an old Sequencher file

Phillip San Miguel via autoseq%40net.bio.net (by pmiguel At purdue.edu)
Wed Nov 1 19:50:28 EST 2006

Martin MOKREJŠ wrote:
> Hi,
>   I am not sure whether this is the right list to post such user
> problems ... but I have received a file with some old sequence data
> created on an old Mac around 1998/2000, maybe with Sequencher but
> who knows. Definitely, current versions of Sequencher cannot open
> it. Inspection of the file contents reveals at least a part of the
> file contains words like "Gel FilePDMF", "ABI1", "36-well
> sharks-tooth", "377 MODL", "377MTXF", "Mac IIsi", etc. Is there
> anybody on the list who could successfully open the file from
> http://www.iresite.org/~mmokrejs/pSN_1-814 in any software and get
> at least the sequences out (if not the annotation)?
>>From the postscript file placed at
> http://www.cs.cmu.edu/~genome/Papers/clark.html I actually think it
> is a Gel File from ABI Prism 377. Still, I don't have the software
> to open it. I would be very very gratefull for any help.
> Martin
If it is a .gel file then the actual sequence text and even the trace 
file would not have been generated yet. The gel file needs to be 
processed into a .abi file. Anyone have a 377 console they could give 
this a shot on? If not you could try looking for an old program, I think 
it was called "bass", that ran under unix but could track gel files.

Purdue Genomics Core Facility

More information about the Autoseq mailing list

Send comments to us at biosci-help [At] net.bio.net