IUBio

[Automated-sequencing] ABI 3100 10X Running Buffer

Michael jmichaeldavenport at gmail.com
Mon Jul 25 17:19:15 EST 2005


I have read that the ABI 10X Running Buffer for the 3100 contains 100mM
TAPS and 1 mM EDTA, but I have not found a complete ingredients list.
Does anyone know of the complete protocol for making this running
buffer from dry ingredients?

As a side note:  I think it is both callous and greedy of ABI to sell
25mL of 10X buffer for $100 per bottle, especially at the rate in which
it is discarded.



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