Paul,
I have, but never tried below 1/2 reactions. Amersham's polymerase/chemistry
allows much quicker thermal cycling (extensions are only 1min instead of 4 min).
But I think their polymerase is not as thermal stable. So while BD runs fine for
99 cycles--at least in the presence of 5% DMSO, I think the Amersham chemistry
will not.
Also BD v3.1 under version 2.0 of data collection in our hands just gives
better results than Amersham's chemistry.
If you are looking to save money, why not drop to 1/16th reactions with the Big
Dye--that works fine.
Phillip SanMiguel
Purdue Genomics Core Facility
Paul Shinn wrote:
> Does anyone use Amersham ET Terminators for sequencing plasmid on the
> 3700? We've been using BigDye for years but I can save a considerable
> amount if I can switch to the ET Terminators. I need to dilute them to
> 1/6rxn to save any money (I do 1/8 BD now). Can you dilute it this low
> for the 3700? Are you happy with the quality?
>> Thanks, Paul
>>> ---
> Paul Shinn pshinn at neomorph.salk.edu> SIGnAL http://signal.salk.edu> Sequencing Coordinator http://genome.salk.edu> (858) 453-4100 ext 1796
>
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