Amersham ET terminators for 3700

Paul Shinn pshinn at force.stwing.upenn.edu
Mon Oct 13 11:09:08 EST 2003

Verena Bohle <vbohle at oncoscreen.com> wrote:
: Has anyone tried to reduce the extension time using BigDye? What did you no=
: tice then?

    When I get the time I will have to try Phillip's lower BD dilution.

Currently I am doing a 2 minute extension with BDv3.1 on plasmids.  I
do at least 50 cycles and normally let it go 99 cycles overnight and
run them in the morning on the 3700.  They go out to 700-750 bases and
I don't see any problems.


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