ITS duplicate bands

Dr N.I.Leaves nleaves at hgmp.mrc.ac.uk
Fri Jul 4 16:46:14 EST 2003

cut them out of the gel and sequence ?


On 3 Jul 2003, Rick McCourt wrote:

> Any advice on obtaining sequence for multiple ITS bands?  Other than
> cloning?  Touchdown PCR and changing the annealing temp doesn't get rid
> of one of two consistent bands.
> Rick McCourt
> Botany
> Academy of Natural Sciences
> 215-299-1157
> ---

Dr N I Leaves
Sequencing Specialist and Key Account Manager
MRC Geneservice
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email: nleaves at geneservice.mrc.ac.uk
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