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Mathew Nightingale testwood at hotmail.com
Thu Feb 27 09:28:07 EST 2003


My company has recently purchased a 3100 for DNA sequencing, and i was
wondering if anyone has any tips for trying to reproduce the ABI's advert of
1000bp :)

Can it really be done with routine samples? We have tried the 50cm array
with very good results, but the 80cm doesnt appear so good, infact the 80cm
array data is worse then the 50cm, even with the same samples!

Any tips on DNA cleanup, analysis setting or run conditions would be great.

Many thanks


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