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Cuvette temp on 3700

Dr N.I.Leaves nleaves at hgmp.mrc.ac.uk
Fri Feb 21 09:32:48 EST 2003

Hi Julie,

changing the cuvette temp is easy. But first you need to decide what
tempreature to change it to. Run a spatial calibration at different
temperatures to try and counteract the left to right variation.

Then depending on the collection softeware you're running you change the
cuvette temp directly in the run module or you change it under "set
cuvette temp" on the main window.

Have a look in the pdf manual for details of how to do this.


On 18 Feb 2003, Julie McCollum wrote:

> Howdy!
> We are running Multi-color Fingerprinting (sort of like SNaPshot - uses
> the same reagents) on our 3700 and we're seeing a signal variation
> across the capillaries.  The SNaPshot protocol manual tells me to
> adjust the run temp and voltage to counteract this problem.  I did
> that, and it helped a little, but we're still seeing a large variation.
>  The book also says that this could be caused by the cuvette temp not
> being optimized, and to test cuvette temp in increments of 5 deg C
> (from 35 - 50) to find the best temp.  Does anyone know how to do this?
>   I looked in the 3700 manual, and it says "We recommend that you not
> change this value unless advised otherwise by PE Biosystems Support
> personnel, but doesn't tell me how to do it. I tried to call ABI, but
> they're off today for President's Day.
> I know how to change stuff in modules, but can't for the life of me
> figure out how to change the cuvette temp.  Any suggestions (other than
> wait 'til tomorrow)?
> Thanks!
> Julie
> --
> Julie McCollum
> julie at sorggenome.tamu.edu
> College Station, TX

Dr N I Leaves
Sequencing Specialist and Key Account Manager
MRC Geneservice
Babraham Bioincubator, Babraham
Cambridge, CB2 4AT, UK
tel: 01223 494557 (office) or 01223 494541 (lab)
mob: 07968 227989        fax: 01223 494512
email: nleaves at geneservice.mrc.ac.uk
web: http://www.hgmp.mrc.ac.uk/geneservice/

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