> My question is: Is this happening because of the succesive acid washes
> or is the spatial calibration doing anything to optimize the output
> from indivual capillaries, that are out of alignment.
A spatial for each capillary will pass or fail based upon signal
intensity. I don't think that at the level of passing or failing a spatial
the alignment of the array should be an issue, although it is used to
subsequently calibrate the bin for each capillary <can anyone confirm
this?>.
I think that you have un-blocked some capillaries either by running
another acid wash or by just using the array in the weeks between the 2
spatials. Irrespective of whether a capillary is marked as blocked it will
be filled with polymer and pressurized every time you make a run.
Good luck
Nick
end
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Dr N I Leaves
Mouse Sequencing
MRC HGMP Resource Centre
Hinxton
Cambridge CB10 1SB
tel: 01223 494557 (office) or 01223 494541 (lab)
email: nleaves at hgmp.mrc.ac.uk
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