Erin, et al.
Our protocols for diluting BD v3 and automated
pipetting on the Hydra96 are at URL:
http://www.genome.ou.edu/PredispensedSeqMixBDv3.html
Cheers,
--bruce
Erin Peden wrote:
> Hello All,
> I do a small amount of sequencing from PCR products until now I
> have been using applied biosystems Big Dye v2.0. It seems I will have to
> get Big Dye v3.0. I was wondering if I would be able to dilute the newer
> chemistry the same way I was diluting before. Currently I use 1ul of the
> BigDye Mix, 2ul of a dilution buffer, 0.5ul of 10uM sequencing primer, and
> 3.5ul of PCR product. Any thoughts would be greatly appreciated. Thanks.
>> Erin Peden
>> 4439 Tupper Hall
> 1 Shields Ave
> Davis CA 95616
> 530-754-6093
>> ---
>>
--
*************************************************************************
Bruce A. Roe, Ph.D George Lynn Cross Research Professor
Department of Chemistry and Biochemistry
620 Parrington Oval, Rm 311
University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610; FAX: (405) 325-7762; e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************
---
