We are doing Gene Scan on our ABI 3100, specifically SNaP Shot for SNP
typing and have noticed that when we combine our Liz size standard with our
reaction in HiDi formamide, the liz signal is much diminished compared to
liz and formamide alone. What is quenching the liz signal? Anyone know the
emission/excitation characteristics of Liz? Our product signals are fine.
Thanks for the help. Please respond directly to mkeller at mail.tju.edu
Margaret A Keller, PhD
Cardeza Foundation for Hematologic Research
Jefferson Medical College
Thomas Jefferson University
Philadelphia PA
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