Using BIGDYE on the MegaBACE 1000

Dr N.I.Leaves nleaves at hgmp.mrc.ac.uk
Thu Mar 14 05:10:39 EST 2002

You need to get hold of a big dye filter set. I know filters have been
made for bd1 and 2 but dont know whether there any any yet for bd3. What
do Amersham say?


On 13 Mar 2002, Monette Aujay wrote:

> I am looking for a protocol using BigDye chemistry on a Megabace 1000.  We
> currently use Amersham's Dynamic ET terminators on our Megabace with great
> success but would like to compare the two chemistries for some of our
> difficult templates.  I assume we need to change mobility files, etc. but
> Applied Biosystems refuses to supply information regarding BigDye chemistry
> on Amersham instruments.
> Thanks in advance,
> Monette Aujay
> Rigel Pharma.
> maujay at rigel.com
> ---

Dr N I Leaves
Mouse Sequencing
MRC HGMP Resource Centre
Cambridge CB10 1SB
tel: 01223 494557 (office) or 01223 494541 (lab)
email: nleaves at hgmp.mrc.ac.uk

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