We did sequencing on megabace 1000 to discover new SNPs. But much more
“indel” could be found especially in the first 150base per
read than ABI 377. I checked the chromatogram : the full sequence was
very good but the peaks at the indel position were often ambiguous.
Does anyone know why this happened? The problem is the machine or the
basecaller?
lijx at genomics.org.cn
Beijing Genomics Institute
Beijing Airport Industrial Zone B6
Beijing, P.R.China, 101300,
Tel: 0086-10-8048-1778(Office)
http://www.genomics.org.cn
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