Hi Tom,
We have successfully used the TempliPhi kit directly from both fresh
overnight bacterial cultures and thawed glycerol stocks. We are using
the 10,000 reaction kit ourselves, but you should be able to purchase
100 and 500 rxn kits as well.
Best Regards,
Glenis
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Glenis Wiebe
Facility Leader, DNA Sequencing
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 Dresden, Germany
Tel: +49-351-210-2628
Fax: +49-351-210-2000
e-mail: wiebe at mpi-cbg.dehttp://www.mpi-cbg.de
Tom Knight wrote:
>> Has anyone tried the Templiphi DNA prep system from un-purified cell
> colonies/cultures? Has anyone been able to buy less than the 10,000
> reaction size enzyme mix?
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