We are trying to sequence plasmid DNA the MegaBACE 1000.
In about 50% of the times we get a signal that seems to be
overloaded.
For all the Plasmid DNA samples we use 200ng of DNA (injection 3kV,
45 sec, denaturation before inejcting: 80 degrees C, 1-2 min.).
Amersham tells us that it can't be due to overloading.
Does anyone knows what is really the problem?
Mathieu Platteel
_______________________________________
\ Ing. M.Platteel
/ Dept. of Pathology and labmedicine,
\ Section Medical Biology,
/ Laboratory for Molecular Biology,
\ Sequence Unit
/ Tel: 050-3619387
\ M.Platteel at med.rug.nl
/_____________________________________
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