IUBio

DNASeq - MegaBACE matrix

jxli sonytoad at hotmail.com
Thu Feb 21 06:07:05 EST 2002


I think the full filled volume is enough to use at least two times and
sometimes three times. It is difficult to collect small amount because
it's too sticky.
jxli

Laurence Hall <hall at RUBICONGENOMICS.com> wrote in message news:<a4rl1r$2sm$1 at mercury.hgmp.mrc.ac.uk>...
> Glenis,
>
>  I posed this very same question to Amersham.
>
> The answer is you cannot because you run a serious risk of introducing
> air bubbles by injecting 'spent' LPA matrix
>
>
> Laurence Hall
>
> -----Original Message-----
> From: Glenis Wiebe [mailto:wiebe at mpi-cbg.de]
> Sent: Saturday, February 16, 2002 10:06 AM
> To: ABRF Newsgroup; autoseq at net.bio.net
> Subject: DNASeq - MegaBACE matrix
>
>
> I would like to hear from MegaBACE users regarding matrix usage.
> Specifically, I am interested to know if anyone re-uses the matrix that
> is left in the pre-filled tubes after a run, and if so:
>
> 1) Can the volume be below the recommended volume of 0.7 ml?
> 2) Can small amounts be combined together for re-use?
> 3) How many times can this be done?
>
> Thanks in advance
>
> Glenis
>
> --
> Glenis Wiebe
> Facility Leader, DNA Sequencing
> Max Planck Institute of Molecular Cell Biology and Genetics
> Pfotenhauerstrasse 108
> 01307 Dresden
> Germany
>
> Tel: +49-351-210-2628
> Fax: +49-351-210-2000
> e-mail: wiebe at mpi-cbg.de
> webpage: www.mpi-cbg.de
>
> ----------------------------------------------
> The views and ideas posted on the ABRF Electronic Discussion List are
> those of the participants and no responsibility can be taken by the
> ABRF.
> To unsubscribe yourself from this mailing list, send a blank email to:
> <list_leave_ABRF at list.abrf.org>
> To change the address at which you receive the ABRF List just
> "unsubscribe" using the old email account  and "subscribe" using the new
> email account.
> To post anonymously, send a message to ombudsmn at aecom.yu.edu (note
> spelling).
> Please do not set "autoresponders" to reply to this list.  A better
> solution is to "suspend" your subscription when you leave and
> "unsuspend" when you return.
> For these and other list commands, send a blank email to:
> <list_help_ABRF at list.abrf.org>
> For posting guidelines and other information see our web site at
> http://www.abrf.org/index.cfm/page/discList/ListServe.htm
> For questions and problems, please send a message to
> listmaster at list.abrf.org
> You are currently subscribed as: hall at rubicongenomics.com
>
> ---

---




More information about the Autoseq mailing list

Send comments to us at biosci-help [At] net.bio.net