DNASeq - MegaBACE matrix

jxli sonytoad at hotmail.com
Thu Feb 21 06:07:05 EST 2002

I think the full filled volume is enough to use at least two times and
sometimes three times. It is difficult to collect small amount because
it's too sticky.

Laurence Hall <hall at RUBICONGENOMICS.com> wrote in message news:<a4rl1r$2sm$1 at mercury.hgmp.mrc.ac.uk>...
> Glenis,
>  I posed this very same question to Amersham.
> The answer is you cannot because you run a serious risk of introducing
> air bubbles by injecting 'spent' LPA matrix
> Laurence Hall
> -----Original Message-----
> From: Glenis Wiebe [mailto:wiebe at mpi-cbg.de]
> Sent: Saturday, February 16, 2002 10:06 AM
> To: ABRF Newsgroup; autoseq at net.bio.net
> Subject: DNASeq - MegaBACE matrix
> I would like to hear from MegaBACE users regarding matrix usage.
> Specifically, I am interested to know if anyone re-uses the matrix that
> is left in the pre-filled tubes after a run, and if so:
> 1) Can the volume be below the recommended volume of 0.7 ml?
> 2) Can small amounts be combined together for re-use?
> 3) How many times can this be done?
> Thanks in advance
> Glenis
> --
> Glenis Wiebe
> Facility Leader, DNA Sequencing
> Max Planck Institute of Molecular Cell Biology and Genetics
> Pfotenhauerstrasse 108
> 01307 Dresden
> Germany
> Tel: +49-351-210-2628
> Fax: +49-351-210-2000
> e-mail: wiebe at mpi-cbg.de
> webpage: www.mpi-cbg.de
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