On 17 Dec 2002, Phillip San Miguel wrote:
> I've been running my 3700 for a couple of years for sequencing only. But now
> I'm ready to start fragment analysis as well. Currently I run POP5. I understand
> that I need to switch back to POP6 for fragment analysis. Does anyone switch
> back and forth between these two polymers frequently? Any problems? How about
> spectrals and spatials? Do spectrals and spatials run under POP5 work for POP6?
>
For framgent analysis you will need to run a new matrix (spectral), what
you choose will depend upon your dye choices in the PCRs. The spatials
will be fine. When you change polymer, I have been told that a POP to
water, then water to POP service modules are the best way to go.
nick
end
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Dr N I Leaves
Genomic Sequencing
MRC HGMP Resource Centre
Hinxton
Cambridge CB10 1SB
tel: 01223 494557 (office) or 01223 494541 (lab)
email: nleaves at hgmp.mrc.ac.uk
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