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primer designe

Marta martaklu at yahoo.com
Fri Apr 19 14:34:39 EST 2002

I am a 5 th year biology student and I am trying to designe primers
for PCR reaction using the Primer3 software. Could anymore tell me if
it matters which strand I designe the primers?
If I subbmit the 3'-5' sequence to the program (eventhough it asks for
5'-3' sequence)will the primers still amplify the desired fragment of

Than you


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