filters vs gratings

MES walkmori at sover.net
Mon Apr 1 09:15:26 EST 2002

Thank you for your input.

A few more questions if you don't mind.

1. Is there a rule of thumb on minimum dye-stokes-shift a grating-based
instrument can handle?

2. Is there a rule of thumb on minimum sample volume a grating based
instrument can handle?

3. Is there a drop off in demand for sequencers?

"David C. Fritzinger" <dfritzin at nospam.hotmail.nospam.com> wrote in message
news:a898qp$5rl$1 at mercury.hgmp.mrc.ac.uk...
> In article <a87bvg$hmo$1 at mercury.hgmp.mrc.ac.uk>, MES
> <walkmori at sover.net> wrote:
> > Recently I have been told that companies like Applied Biosystems are
> > from optical filters to gratings for spectral filtering.
> >
> > Is it possible to sequence with a grating based instrument?
> >
> > Is the near band rejection high enough?
> >
> > Is the optical energy throughput high enough?
> Actually, I believe that, ever since the 377, ABI has been using
> gratings for spectral filtering. That's why they call their instruments
> "Prism". It is also why it is so simple to use a number of different
> dye sets in ABI insturments.
> I believe the answer to all your questions are yes. Both types of
> insturments have advantages. I believe the MD machines, which use
> filters and a confocal microscope for detection are more sensitive,
> while ABIs insturments are easier to set up for different dye sets, and
> easier to use for more than 4 dyes.
> Hope this helps.
> Dave Fritzinger
> >
> >
> > ---
> ---


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