Samantha,
Are you using the .2 mm or .4 mm combs and spacers. we used to use the
.4 mm combs and had similar problems with leaking. We switched to the
.2 mm combs and found that they tend to not leak as much or as often We
also clamp along the top of the gel where we are placing the combs.
Meaning that the binder clamps are placed so the put pressure where the
casting part of the comb is placed to make a tighter fit.
Also over time the teeth tend to wear out as you may have noticed and
actually should be thinner than the casting side of the comb to ensure
an easier fit.
Hopefully this will help
Jason Klein
Myriad Genetics
Diagnostics Group Leader
Samantha Donninger wrote:
>> Hi there,
>> We're experiencing a problem with the sharkstooth combs for the ABI
> 377, and any suggestions would be much appreciated.
>> We're finding that our wells have started leaking when we load the
> gel, and the problem seems to be that the well formed is perhaps too
> wide, making the teeth loose in the well. We don't get acrylamide
> in the well, so it doesn't seem to be a problem with the clamping.
>> New combs work fine, which suggests that there might be some build-up
> on the older combs?
>> Has anyone experienced this? If so, any suggestions on the best way
> to "strip" the combs?
>> Many thanks,
> -Samantha Donninger
>> ------------------------------------------
> MRC Human Genetics Research Unit
> Division of Human Genetics
> Department of Clinical Laboratory Sciences
> University of Cape Town
>> email: st at cormack.uct.ac.za> ph: (+27 21) 406 6456
> fax: (+27 21) 488 0906
>> ---
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