We have now switched to disposable paper combs and appropriate well former
(both supplied by ABI) on our 377 and have found them to work very well.
Mark
"Samantha Donninger" <st at cormack.uct.ac.za> wrote in message
news:9oetv0$hf0$1 at mercury.hgmp.mrc.ac.uk...
> Hi there,
>> We're experiencing a problem with the sharkstooth combs for the ABI
> 377, and any suggestions would be much appreciated.
>> We're finding that our wells have started leaking when we load the
> gel, and the problem seems to be that the well formed is perhaps too
> wide, making the teeth loose in the well. We don't get acrylamide
> in the well, so it doesn't seem to be a problem with the clamping.
>> New combs work fine, which suggests that there might be some build-up
> on the older combs?
>> Has anyone experienced this? If so, any suggestions on the best way
> to "strip" the combs?
>> Many thanks,
> -Samantha Donninger
>>> ------------------------------------------
> MRC Human Genetics Research Unit
> Division of Human Genetics
> Department of Clinical Laboratory Sciences
> University of Cape Town
>> email: st at cormack.uct.ac.za> ph: (+27 21) 406 6456
> fax: (+27 21) 488 0906
>> ---
>
