ABI 377 combs

Samantha Donninger st at cormack.uct.ac.za
Fri Sep 21 02:21:34 EST 2001

Hi there,

We're experiencing a problem with the sharkstooth combs for the ABI 
377, and any suggestions would be much appreciated.

We're finding that our wells have started leaking when we load the 
gel, and the problem seems to be that the well formed is perhaps too 
wide, making the teeth loose in the well.  We don't get acrylamide 
in the well, so it doesn't seem to be a problem with the clamping.

New combs work fine, which suggests that there might be some build-up 
on the older combs?

Has anyone experienced this?  If so, any suggestions on the best way 
to "strip" the combs?

Many thanks,
-Samantha Donninger

MRC Human Genetics Research Unit
Division of Human Genetics
Department of Clinical Laboratory Sciences
University of Cape Town

email:  st at cormack.uct.ac.za
ph:  (+27 21) 406 6456
fax:  (+27 21) 488 0906


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