Hello,
I am performing DNA Sequencing of methylated bases on
my ABI 377. I got phenomena of signals shooting up
where no particular base present (e.g C or G) and
having target bases shifted. I've asked ABI for
advice, ending up adjusting the basecaller's rfu ratio
setting and no further suggestion came out. However,
it seems that I still don't get any well optimised
electropherogram results.
Would there be anyone who has done bisulphite modified
DNA sequencing and could comment on optimising this
kind of research work?
Jeff
__________________________________________________
Do You Yahoo!?
Make a great connection at Yahoo! Personals.
http://personals.yahoo.com
---
