sequencing post-lineraization

SMarsh3807 smarsh3807 at aol.com
Mon Oct 15 17:32:04 EST 2001

>Life Technologies now Invitrogen do three sequencing reaction 
>additives/buffers termed Sequencer Rx buffers A, E and F.

We have tried them on gc rich templates and they work well for us. We don't
supply them, the customers bring it with their samples. We have not ried it for
BAC's yet. We get decent results using ABI's BD v2.0 and the protocol for
cycling that they recomend inthe protocol book that comes with the kit.
Steve Marsh
DNA Seq Core Director Caltech


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