KS primer quality in high throughput sequencing

Jaswinder Khattra jkhattra at bcgsc.bc.ca
Mon Nov 26 18:38:48 EST 2001

Seeking opinions on the quality of sequence obtained with the KS primer (TCGAGGTCGACGGTAT) using Dye Terminator cycle sequencing chemistry and capillary electrophoresis. My specific situation involves pPCR-Script Cam SK(+) vector with blunt-ended microbial genomic DNA inserts.
Thank you,

Jaswinder Khattra
Genome Sequence Centre
BC Cancer Agency
600 West 10th Ave.
Vancouver BC V5Z4E6

604-877-6098 x2548
fax 877-6085

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