The software for subtracting a reference sequence trace from another
sequence trace is trace_diff.
See the following web site:
http://www.angis.org.au/Staden/manual/mutations_2.html. The program is
part of the Staden package.
Myriad Genetics using a similiar program for their mutation detection in
BRCA1 and BRCA2 however it is proprietary.
>Duncan Clark wrote:
>> In message writes on the Mon, 5 Nov 2001
> >Is there any software that allows you to separate two sequences from
a
> >mixed template reaction?
>> Well think about it. How on earth are you going to be able to tell
which
> base belongs to which sequence in the mixture at any one position.
>> Imagine you could get an A and C then an A and T in the next position.
>> Software could assign AA, AT, CA or CT. Keep doing this at every
> position and you will have hundreds of possible sequence combinations.
> If you know the sequence of one of the mix DNA's then it will help,
> otherwise I would start again.
>I understand that but, as I stated in the original post, I was just
>relaying a question from a colleague.
>There is supposedly software that does this. She had read about it in a
>paper. As you say, presumably it uses a reference trace from a known
>sequence and subtracts that to leave the other trace.
>Kevin.
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