Separating mixed sequence traces

Robert Chadwick rchadwick at genomichealth.com
Wed Nov 7 11:20:37 EST 2001

The software for subtracting a reference sequence trace from another
sequence trace is trace_diff.  
See the following web site:
http://www.angis.org.au/Staden/manual/mutations_2.html.  The program is
part of the Staden package.
Myriad Genetics using a similiar program for their mutation detection in
BRCA1 and BRCA2 however it is proprietary.

>Duncan Clark wrote: 
> In message writes on the Mon, 5 Nov 2001 
> >Is there any software that allows you to separate two sequences from
> >mixed template reaction? 
> Well think about it. How on earth are you going to be able to tell
> base belongs to which sequence in the mixture at any one position. 
> Imagine you could get an A and C then an A and T in the next position.

> Software could assign AA, AT, CA or CT. Keep doing this at every 
> position and you will have hundreds of possible sequence combinations.

> If you know the sequence of one of the mix DNA's then it will help, 
> otherwise I would start again. 
>I understand that but, as I stated in the original post, I was just 
>relaying a question from a colleague. 
>There is supposedly software that does this. She had read about it in a

>paper. As you say, presumably it uses a reference trace from a known 
>sequence and subtracts that to leave the other trace. 


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