genotyping: DNA in PCR

Justin Hopkins justin.hopkins at kcl.ac.uk
Fri Mar 30 07:44:44 EST 2001

Routinely 12.5ng of DNA in 5uL works well for STR PCR with 30 cycles.

You could quite likely use less but would depending on the quality of your
DNA prep.

Lynn Petukhova wrote:

> Hello everyone:
> I have a quick question for people doing Genescan:
> How much DNA do you use in your PCR?
> We use 20ng in a 5uL reaction and I would like to reduce this.

Justin Hopkins
Cancer Genetics Laboratory
8th Floor, Guy's Tower
Guy's Hospital
London SE19RT


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