I am trying to sequence a 700 bp PCR fragment. I cannot seem to clone
this fragment because it appears to be susceptible to rearrangements. I
have managed to sequence about half of it, then the sequence just
becomes illegible. Although I have used a number of forward and reverse
primers, the sequence stops in the same place, at what appears to be a C
rich region. I have been using the ABI system and dye terminators.
Does anyone have any suggestion as to how I can sequence through the
last 300-400 bp of this sequence?
Sabi na Belli (PhD)
Molecular Parasitology Unit
University of Technology, Sydney