IUBio

PE biosystems version 3 sequencing kit

Joanne Gilfillan jag at cormack.uct.ac.za
Thu Jul 19 04:56:59 EST 2001


PLEASE HELP!!!

We recently tried out the Big Dye Terminator Kit Version 3 on our ABI 
377 sequencer.  We experienced an offset/shift of the GTP in relation 
to the other bases, creating problems with the base calling. Version 
1 of this kit gave us no problems at all. Has anyone else also found 
this, and if so, are there any ideas on how to remedy this problem? 
Does anyone know whether version3 has been tested extensively on 
gel-based systems? 

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