Phred Quality analysis

Mark T Jung MARK.T.JUNG at USA.dupont.com
Tue Jul 17 05:39:34 EST 2001


     We have been using phred for about a year now to QC our sequencing
results and have found this to to be
very useful.  My impression is that most others would agree with this.  We
did a direct comparison a few months
ago with Trace Tuner (Paracel) and found we liked the phred quality
assignments better.  Paracel claims a 10%
greater read length on 3700 data; we could not confirm this on our 3700
data.  We use phred's trimming feature to
trim our data.  E-Mail me directly if you want specifics regarding how we
do the analysis.

Mark Jung

Tony <darthvaderscat at hotmail.com> on 07/12/2001 10:06:25 AM

To:   autoseq at net.bio.net
cc:    (bcc: Mark T Jung/AE/DuPont)
Subject:  Phred Quality analysis

Has anyone out there has any sucess storied with long term monetering
of sequence quality using the phred<20 scores for the 3700?
I am currently trying to get a quality system based on phred scores
internally validated and I would like to hear from people who have
done the same thing and how they got on. Amongst my many problems are
assigning cut-offs.
Thankyou in advance


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