Tony,
We have been using phred for about a year now to QC our sequencing
results and have found this to to be
very useful. My impression is that most others would agree with this. We
did a direct comparison a few months
ago with Trace Tuner (Paracel) and found we liked the phred quality
assignments better. Paracel claims a 10%
greater read length on 3700 data; we could not confirm this on our 3700
data. We use phred's trimming feature to
trim our data. E-Mail me directly if you want specifics regarding how we
do the analysis.
Mark Jung
Tony <darthvaderscat at hotmail.com> on 07/12/2001 10:06:25 AM
To: autoseq at net.bio.net
cc: (bcc: Mark T Jung/AE/DuPont)
Subject: Phred Quality analysis
Has anyone out there has any sucess storied with long term monetering
of sequence quality using the phred<20 scores for the 3700?
I am currently trying to get a quality system based on phred scores
internally validated and I would like to hear from people who have
done the same thing and how they got on. Amongst my many problems are
assigning cut-offs.
Thankyou in advance
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