The new dGTP BigDye kit should have very good signal at the beginning of your
sequence.
Our experience is, if you have a very good and clean template, even using
dRhodamine or BigBye can read right from the first nucleotide after the primer
DNA Sequencing Facility wrote:
> I have a customer who would like to be able to read within 15 bases of the
> end of the primer. I have a 373 ABI sequencer. It seems that there is
> some variety in how close to the primer the sequencer will read. Any
> suggestions?
>> Thanks,
> Paula
>> ---
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Y.F.LEUNG
Department of Ophthalmology and Visual Sciences
Chinese University of Hong Kong
3/F Hong Kong Eye Hospital
147K Argyle Street, Hong Kong
Tel: (852) 27623152 Fax: (852) 27159490
email: leungyukfai at hotmail.com, yfleung at cuhk.edu.hk
URL: http://i.am/yfleung (My functional genomics home)
