Sequencing close to the primer

Y.F.LEUNG yfleung at cuhk.edu.hk
Thu Feb 1 08:22:52 EST 2001

The new dGTP BigDye kit should have very good signal at the beginning of your
Our experience is, if you have a very good and clean template, even using
dRhodamine or BigBye can read right from the first nucleotide after the primer

DNA Sequencing Facility wrote:

> I have a customer who would like to be able to read within 15 bases of the
> end of the primer.  I have a 373 ABI sequencer.  It seems that there is
> some variety in how close to the primer the sequencer will read.  Any
> suggestions?
> Thanks,
> Paula
> ---

Department of Ophthalmology and Visual Sciences
Chinese University of Hong Kong
3/F Hong Kong Eye Hospital
147K Argyle Street, Hong Kong
Tel: (852) 27623152 Fax: (852) 27159490
email: leungyukfai at hotmail.com, yfleung at cuhk.edu.hk
URL: http://i.am/yfleung (My functional genomics home)

More information about the Autoseq mailing list

Send comments to us at biosci-help [At] net.bio.net