IUBio

Mixing genescan and sequencing

SMarsh3807 smarsh3807 at aol.com
Tue Aug 21 13:23:17 EST 2001


<< Hi all
does anyone know why you shouldn't run sequencing and genescan on the 3700
at the same time? ABI say not to but tech support don't know why. 
Cheers
Frazer >>


One big thing is they use different dye sets (different spectral calibrations).
You most likely would end up with pull up under your samples which could mess
up the calls in both. 
Steve

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