Hi Sharon
I have used Sequencher for the past 5 or 6 years and find it to be
the fastest, easiest to use alignment software. It can't be beat for speed
and simplicity. I believe if you go the webpage for GeneCodes
http://www.genecodes.com/, you can download a demo copy for a Mac or send
away for a PC version. The Mac version is considerably cheaper than the PC
version. I have worked with the people at Genecodes extensively and their
tech support is great ( although it is so intuitive, you rarely need them).
I would at least check out Sequencher before making any decisions.
Scottie
>Hello Everyone,
>>I have a question about Alignment Software. I am currently sequencing a
>BAC which I am told is about 340 kb. I have it nebulized and subcloned
>into Bluescript. I sequence on a LI COR 4200 and get 700-1100 bp per
>sample. I have been using the LI COR AlignIR program to assemble and
>have crashed the program. I was told to put the contigs into a new
>project to save space, but at this point (85 kb) I have a few 5 kb
>contigs, but most samples haven't matched up yet, so this isn't
>feasible. I have access to the McVector/AssemblyLIGN program (Mac
>version), but the company isn't able to tell me how many samples this
>program can work with or whether a project of this size is feasible.
>>Is it feasible to assemble a project this large on a personal computer?
>Do you think the McVector/AssemblyLIGN program would work? Do you think
>some of the other assembly packages out there would work (such as
>Sequencher?) If so, who is the vendor and what is the cost?
>>Do I need to go to a Unix Platform? I would have access to a UNIX
>system (Phap/Phred) for about $500.00 per year.
>>Any comments or suggestions would be greatly appreciated. Thank you in
>advance.
>>Sharon Minnerath
Scottie Adams aka Pamela Scott Adams
ABRF Fragment Analysis Research Group
http://www.abrf.org
Manager
Molecular Biology Core Facility
Trudeau Institute
100 Algonquin
Saranac Lake, NY 12983
Phone: 518-891-3080
Fax: 518-891-5126
Email: sadams at northnet.orghttp://www.trudeauinstitute.org
---
