hi,
I am doing a whole genome scan for a disease locus. One technical
problem need your help. The problem is that we have a Hybaid PCR
express thermal cycler, the machine is fine except with 96-well
plates. When using 96-well plates, most of the reactions (10 ul) in the
wells along the outside edge of the plate do not work due to
evaporation.
I have tried tape, sealing mate, non of them work. I am sure you have
use 96-well PCR. Could you tell me what kind of machine, plate sealing
method you used. What could be my problem?
Thanks!
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