James,
Since you already have extensive experience with the 377
and obtaining high quality sequencing templates for this slab
gel instrument, I would strongly advise in favor of obtaining
another 377. You can pick up a used one for ~$30,000 and could
use your existing template isolation protocols, etc. Switching
to a capillary instrument will require much additional care in
isolating very clean templates, in addition to the high cost of
the instrument, significant amount of down time for the 3700,
and extreme sensitivity to contaminants in obtaining successful
reads with the capillary instruments.
That's my 2 cents worth.
Cheers,
--bruce
*************************************************************************
Bruce A. Roe, Ph.D George Lynn Cross Research Professor
Department of Chemistry and Biochemistry
University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610; FAX: (405) 325-7762; e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************
You write:
=> Return-path: <owner-autoseq at hgmp.mrc.ac.uk>
=> Received: from mercury.hgmp.mrc.ac.uk (mercury.hgmp.mrc.ac.uk [193.62.192.80])
=> by aardvark.ucs.ou.edu (PMDF V5.2-27 #39381)
=> with ESMTP id <01JW2319TAIU00090H at aardvark.ucs.ou.edu> for
=> broe at aardvark.ucs.ou.edu (ORCPT rfc822;broe at aardvark.ucs.ou.edu); Thu,
=> 2 Nov 2000 08:06:05 CST
=> Received: by mercury.hgmp.mrc.ac.uk (Postfix) id B599C17AC0; Thu,
=> 02 Nov 2000 14:04:44 +0000 (GMT)
=> Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110)
=> id 64A1D17AC1; Thu, 02 Nov 2000 14:04:44 +0000 (GMT)
=> Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001)
=> id 8B64A17AC0; Thu, 02 Nov 2000 14:04:39 +0000 (GMT)
=> Date: Wed, 01 Nov 2000 23:08:31 +0000
=> From: "James M.Schupp" <James.Schupp at NAU.EDU>
=> Subject: sequencer comparison
=> Sender: owner-autoseq at hgmp.mrc.ac.uk
=> To: autoseq at net.bio.net
=> Message-id: <20001102140439.8B64A17AC0 at mercury.hgmp.mrc.ac.uk>
=> Precedence: bulk
=> Newsgroups: bionet.genome.autosequencing
=> Original-recipient: rfc822;broe at aardvark.ucs.ou.edu
=>
=> Hello all,
=>
=> Looking for some advice on purchasing an automated
=> sequencer/genotyper. We are a small one ABI 377 lab looking to add a
=> second machine, thinking about the ABI3100 16 capillary machine. Anyone
=> have some feedback on this machine, the megabace 1000 (there is a 16 and a
=> 48 capillary machine available from APBiotech now) and the MJ Research
=> BaseStation? Concerned about throughput, machine downtime and consumables
=> cost. Not considering the LiCor machine due to different chemistries. Any
=> feedback would be very useful. Thanks
=>
=> Jim Schupp
=> __________
=> / \
=> / /O\ /O\ \
=> / /\ \
=> \ /
=> \ \_____/ /
=> \___________/
=>
=>
=>
=> James M. Schupp
=> Project Director
=> Biology Department, Box 5640
=> Northern Arizona University
=> Flagstaff, AZ 86011
=> 520-523-1120
=> 520-523-7500 FAX
=> JAMES.SCHUPP at NAU.EDU
=>
=>
=> ---
=>
=>
=>
---
