"Leviten, Dina" wrote:
> Stuart,
> I have seen 2 reasons for what you describe. The first is that perhaps
> there are salts competing when injecting--this would mean that all their
> samples tail off. To fix this, a EtOH precipitation usually does the trick.
> For example, we ran tests on different mini-prep kits and found that Promega
> Wizard Plus preps don't sequence well until EtOH ppt'd, however, the Promega
> Wizard Plus SV preps work fine without the extra step. (Qiagen work fine).
> [...]
Do you mean an EtOH precipitataion on your sequencing reaction--or your
template? Wouldn't your dye-terminator clean-up get rid of any residual salt
from the mini-prep?
Phillip San Miguel
Purdue Genomics Core Facility
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