3700 sequencing

Stuart Bayliss stuart.bayliss at csc.mrc.ac.uk
Fri May 12 03:36:11 EST 2000


I have recently begun to use a 3700 for sequencing in a core facility.
Certain users have noticed samples in which data quiality tails off quite
early (200-300 base region).  These users usually obtain >650 bases when
the samples are run on a 377.

I was thinking it may be due to the electrokinetic injection preferentially
injecting salts etc into the capillary ahead of the sample in turn leading
to low signal/longer fragments missing...which maybe wouldn't cause undue
problems on a gel system.

Has anyone seen such cases?  I'd appreciate any help you could give.


Stuart Bayliss,
Services Co-ordinator,
Genetics Core Facility,
MRC Clinical Science Centre,
Clinical Research Building,
Hammersmith Hospital,
Du Cane Road,
W12 0NN

email:  stuart.bayliss at csc.mrc.ac.uk
Tel (office):   0208 383 8305
Tel (lab):      0208 383 3181
Fax:            0208 383 8338


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