Hi,
I have recently begun to use a 3700 for sequencing in a core facility.
Certain users have noticed samples in which data quiality tails off quite
early (200-300 base region). These users usually obtain >650 bases when
the samples are run on a 377.
I was thinking it may be due to the electrokinetic injection preferentially
injecting salts etc into the capillary ahead of the sample in turn leading
to low signal/longer fragments missing...which maybe wouldn't cause undue
problems on a gel system.
Has anyone seen such cases? I'd appreciate any help you could give.
Regards,
Stuart.
Stuart Bayliss,
Services Co-ordinator,
Genetics Core Facility,
MRC Clinical Science Centre,
Clinical Research Building,
Hammersmith Hospital,
Du Cane Road,
London.
W12 0NN
email: stuart.bayliss at csc.mrc.ac.uk
Tel (office): 0208 383 8305
Tel (lab): 0208 383 3181
Fax: 0208 383 8338
http://146.179.68.156
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