>Dear DNAseq'ers,
>>one additional question for the 3700 specialists:
>>When switching to v1.1 and POP5, were does base calling start (in
>bases away from the primer)? On the runs I have seen, the first about
>70 or so bases are lost due to mobility shifts. Our single 3700 is
>now running with v1.1, but we still use POP6. Has the problem been
>solved or is it anyhow only roumors?
>>Thanks, Bernd
Hello!
Have there been any further conclusions about what this mobility shift
is? I have been trying to optimize our new (and only) 3700, and
regularly see these mobility shifts in the first 70 bases. When I
called tech support, they stated that they had never heard of this
problem and were not sure of what could cause it. So far, I have set up
experiments to test concentration of DNA in my reactions, and this
mobility problem seems to be independent of template concentration. I
have also experimented with different injection buffers and amounts, and
have seen no effect on the mobility shift in any of my trials. And, I
do use an increased injection time of 60 seconds (a carryover from the
signal intensity problems we encountered after setup and before laser
alignment). I have not tested whether these shifts are related to
injection time.
Any ideas?
Thanks so much!
Stephanie
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