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PCR sequencing

Cameron McCrae cameron.mccrae at anu.edu.au
Tue Jul 18 23:40:48 EST 2000


Dear All, when sequencing PCR products I have found that some sequences
show little or no signal for the first 10-30 bases and then seem to
gradually make an appearance. Doesn't happen to all products on a gel, but
in an array of 96 perhaps 10-25% are affected. Any clues?

Cheers,
Cameron



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