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Big dye sequencing

Frazer Murray frazer.murray at bbsrc.ac.uk
Tue Jul 11 05:58:40 EST 2000

A quick question.
How important is it to cool the sequencing block to 4oC after the final
step in the sequencing reaction? Could the incorporated dyes be degraded
if it is not cooled? I am asking after an accident where the heated lid
did not switch off after the final reaction step and so remained at 60oC
for several hours after. The reactions had all failed when run on a gel.
Many thanks

Frazer Murray
Roslin Institute
frazer.murray at bbsrc.ac.uk

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