Happy y2k,
MTBE aka Modified Tris-borate-EDTA buffer, is made by
doubling the Tris and halfing the borate from
what was in typical TBE when it was initially
introduced. ABI recommends slightly different
amounts and we've pretty much stuck with what
ABI recommends for the 377's
10x MTBE (Modified Tris-borate-EDTA buffer
1.3 M Tris, 0.4 M Boric acid, and 0.02 M EDTA
in double distilled water.
162 g Tris base
27.5 g Boric acid
9.3 g Na2EDTA
ddH2O to 1 L
10X ABI TBE:
216 g Tris base 108 g
110 g boric acid 55 g
16.6 g EDTA 8.3 g
Add ddwater to 2 L Add ddwater to 1 L
If you don't know about our online protocol book, it's
available at URL: http://www.genome.ou.edu/proto.html
Enjoy,
--b.roe
*************************************************************************
Bruce A. Roe, Ph.D George Lynn Cross Research Professor
Department of Chemistry and Biochemistry
University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610; FAX: (405) 325-7762; e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************
You write:
=> Date: Mon, 17 Jan 2000 22:57:12 +0000
=> From: Mathew Nightingale <mathew at mufc.junglelink.co.uk>
=> Subject: MTBE
=>
=> Hi Everyone,
=>
=> Hope you all had a good Christmas and New Year.
=>
=> I am writing to ask if anyone has ever used MTBE buffer apparently it
=> increases the read length for DNA sequencing.
=>
=> I hope that someone can shed some light on this and maybe offer a protocol
=> to try.
=>
=> Thanks in advance
=>
=> Mathew Nightingale
=>
=>
=>
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