IUBio

Licor Sequencing

Curtis Clevinger clevincc at jmu.edu
Wed Feb 23 15:52:18 EST 2000


I am currently trying to sequence PCR products using custom primers on a
Licor 4000 system.  I have been follwing Licor protocols and the
protocol found in the EXCEL II kit for licor sequencers.  I am not
obtaining good sequences (ie lots of background). Are there any
suggestions from other Licor users, especially from those of you who are
using sequences in plant phylogenetic work.

Thanks for your time

Curtis Clevinger

clevincc at jmu.edu

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