Hi Andrew,
we have encountered the same problem when preparing BygDye sequencing
reactions in 96 well format. We have bought a rotor compatible with a
Heraeus table top centrifuge. In order to make the precipitations work we
needed to increase the centrifugation time to 45 minutes at 1500 g. If you
already have a rotor that fit the 96 well plates, I would suggest that you
try to use that rotor (no of g´s * time should be roughly equal to the
protocol).
Hope this helps,
Dag Ahrén
Microbial Ecology
Lund University
Sweden
Andrew Roger <aroger at IS.Dal.Ca> skrev i
diskussionsgruppsmeddelandet:87eun0$d6m$1 at mercury.hgmp.mrc.ac.uk...
> Hi,
>> We are gearing up to do 96 well minipreps in the 96 well 2.0ml
> "bioblock" (QIAGEN) or similar format. I have been trying to find out
> what microplate centrifuges (or rotors) can be used for both spinning
> down cultures in the 96 well 2.0 ml blocks as well as reach sufficient
> speeds to do ethanol/isopropanol precipitations to clean up big-dye
> terminator reactions. Right now I know the QIAGEN/Sigma 4-15 centrifuge
> + microplate rotor can do this, but it is incredibly expensive (more or
> less double the cost of other desktop microplate centrifuges). Can
> someone point me to other options for microplate centrifuges that would
> be less expensive but can accommodate the 96 well 2.0ml bioblocks AND
> has sufficient speed to do ETOH precipitations in 96 well format? I
> realize that Sorvall has a rotor that will do this also, but its also
> pretty expensive and our Sorval c-fuge is on its last legs.
>> Any help with this would be greatly appreciated.
>> Cheers
> Andrew Roger
> Dept. of Biochem. and Molecular Biol.
> Dalhousie University
> Halifax, N.S.
> B3H 4H7
> ---
>
