Hi,
Sorry, I'm at Oklahoma and guess all the colonies look the same from the
other side of the pond... :-)
The URL on my web site for this info is:
http://www.genome.ou.edu/big_dyes_bact.html
Cheers,
--bruce
*************************************************************************
Bruce A. Roe, Ph.D George Lynn Cross Research Professor
Department of Chemistry and Biochemistry
University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610; FAX: (405) 325-7762; e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************
You write:
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=> 3 Feb 2000 07:30:18 CST
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=> Date: Thu, 03 Feb 2000 11:40:18 +0000 (GMT)
=> From: Dr H Crooke <hcrooke at hgmp.mrc.ac.uk>
=> Subject: Re: Status of direct genomic sequencing
=> Sender: owner-autoseq at hgmp.mrc.ac.uk
=> To: autoseq at net.bio.net
=> Message-id: <20000203122340.CD07317AF1 at mercury.hgmp.mrc.ac.uk>
=> Precedence: bulk
=> Newsgroups: bionet.genome.autosequencing
=> Original-recipient: rfc822;broe at aardvark.ucs.ou.edu
=>
=> In article <875t52$bse$1 at mercury.hgmp.mrc.ac.uk>,
=> ed_taboada at hotmail.com writes:
=> > Hi folks,
=> >
=> > could someone point me to literature outlining the current status of
=> > direct genomic sequencing? I've been out of the sequencing loop for the
=> > last couple of years and would like to know if there are reliable
=> > protocols being used to generate sequence data from genomic DNA using
=> > gene specific primers. We are interested in determining the allele of a
=> > particular gene in a large number of bacterial isolates. We were
=> > leaning towards PCR and hybridization with allele-specific probes but
=> > someone mentioned that we might want to try direct sequencing instead
=> > and we are definitely intrigued. What are people's views on this ?
=> >
=> > thanks in advance,
=> >
=> > Ed
=> >
=> >
=> > Sent via Deja.com http://www.deja.com/
=> > Before you buy.
=> > ---
=> >
=> There are protocols available at Washington (Bruce Roe) I think and Cheryl Heiner (ABI). I find genomic sequencing reasonably reliable but I have way to go to perfect it.
=>
=> Richard
=> r.dobson at microscience.com
=>
=>
---
