We have the same observation .... we load from water, and purify via
self-made G50 colums in 96-well format (spin plates similar to
Eric's).
Good seq-runs, Bernd
>Recently we have noticed that we are getting a large T blob at around
>base 220 and shortly after that a G blob. Sometimes this blob is enough
>to distort base calling, other times it doesn't affect the calls, and
>other times it does not appear. Has anyone else seen this? I am
>guessing it may have to do with our cleanup method. We spin purify our
>plates.
>However, with the rash of annoying 3700 glitches we have had lately, it
>may well have something to do with the instrument.
>Any suggestions?
>Thanks,
>Eric Buxton
>Lexicon Genetics
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