DNAseq: 3700 dye blob question

Bernd Weisshaar weisshaa at mpiz-koeln.mpg.de
Thu Feb 3 07:22:18 EST 2000

We have the same observation .... we load from water, and purify via 
self-made G50 colums in 96-well format (spin plates similar to 

Good seq-runs, Bernd

>Recently we have noticed that we are getting a large T blob at around
>base 220 and shortly after that a G blob.  Sometimes this blob is enough
>to distort base calling, other times it doesn't affect the calls, and
>other times it does not appear.  Has anyone else seen this?  I am
>guessing it may have to do with our cleanup method.  We  spin purify our
>However, with the rash of annoying 3700 glitches we have had lately, it
>may well have something to do with the instrument.
>Any suggestions?
>Eric Buxton
>Lexicon Genetics


PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
Carl-von-Linné-Weg 10
50829 Koeln

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