Ed,
The status of direct higher eucaryotic genomic sequencing is
that you 'can't get there from here'. We have had some success with
genomes as large as E. coli, but that's about it. I would point you
to my web site with the relevant information and references.
http://www.genome.ou.edu/proto.html
Cheers and you'll have to either get the BAC clones or do PCR-based
sequencing.
--bruce roe
*************************************************************************
Bruce A. Roe, Ph.D George Lynn Cross Research Professor
Department of Chemistry and Biochemistry
University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610; FAX: (405) 325-7762; e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************
You write:
=> Date: Mon, 31 Jan 2000 22:16:50 +0000
=> From: ed_taboada at hotmail.com
=> Subject: Status of direct genomic sequencing
=> To: autoseq at net.bio.net
=>
=> Hi folks,
=>
=> could someone point me to literature outlining the current status of
=> direct genomic sequencing? I've been out of the sequencing loop for the
=> last couple of years and would like to know if there are reliable
=> protocols being used to generate sequence data from genomic DNA using
=> gene specific primers. We are interested in determining the allele of a
=> particular gene in a large number of bacterial isolates. We were
=> leaning towards PCR and hybridization with allele-specific probes but
=> someone mentioned that we might want to try direct sequencing instead
=> and we are definitely intrigued. What are people's views on this ?
=>
=> thanks in advance,
=>
=> Ed
=>
=>
=> Sent via Deja.com http://www.deja.com/
=> Before you buy.
=> ---
=>
=>
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