Big Dye Sequencing on 377

Frazer Murray frazer.murray at bbsrc.ac.uk
Mon Aug 28 07:58:21 EST 2000

Hi everyone
has anyone else been having trouble with their sequencing reactions
lately? I've found I've had to increase the amount of sequencing reagent
from 2ul to 3ul (in 10ul total volume) which has considerably increased
costs. I've started on a new library but the old one was showing the same
problem so I don't think that was the cause.
It would be interesting to hear if others had noticed this as well.

Frazer Murray
Roslin Institute
frazer.murray at bbsrc.ac.uk

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