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Loading system for 96 lanes.

Frazer Murray Frazer.Murray at bbsrc.ac.uk
Wed Sep 1 06:23:11 EST 1999


I use the Kloen loader and it is working fine now after a bit of practice.
I had some problems with basecalling though but it was down to loading too
much sample onto the gel (96 lanes are 1/2 size of 48 lanes). I now load
0.8ul of sample (pellet resuspended in 1.5ul) and I'm getting good runs. It
also allows me to repeat the run with the remaining sample if I get a bad
gel.

In article <7qg5aj$pri at net.bio.net>, Louis Geller
<louis_geller at geocities.com> wrote:

> Hi.
>     My lab wants to upgrade to 96 lanes for Sequencing and Genescan
> using an ABI-377-96.  We have the instrument and have been shown the
> protocol by Perkin-Elmer.
>     The problem is what type of loading system to use.  I would like to
> get some information about what multi-channel pipettes people are using,
> what they think of them, and what problems they have encountered with
> the various systems.
>      I would appreciate any help!
> 
>     Louis Geller

-- 
Frazer Murray
Roslin Institute
frazer.murray at bbsrc.ac.uk




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