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96 well gel loader

Stephen R. Lasky srlasky at cellworks.mbt.washington.edu
Mon May 17 17:42:41 EST 1999


"Skip Vaught, Brian Coullahan, Mark Schwartz, and Felisa Blackmer"
wrote:
> 
> Dear fellow DNA sequencers,
> 
> Our Dept. has blessed us with money to purchase 96 well upgrades. ABI
> recommends  2 brands of "expanding/contracting" gel loaders : Kloehn
> Company's Dual position GL/2000 and World Precision Instrument's Gel Mate
> 96.
> 
> Anyone out there using these loaders? I would greatly appreciate your
> critiques, especially concerning ease of use and clogging. A long time ago
> I had a bitter experience with a Hamilton syringe loader and am just
> getting over the pain.
> 
> Best regards,
> 
> Skip Vaught
> DNA Sequencing Service
> University of Arizona
> 
> THE DNA SEQUENCING SERVICE
> UNIVERSITY OF ARIZONA
> 
>   .-. .-.   .-. .-.   .-. .-.   .-. .-.   .-.
>  /|||\|||\ /|||\|||\ /|||\|||\ /|||\|||\ /|||\
> \|||/ \|||\|||/ \|||\|||/ \|||\|||/ \|||\|||/
>  `-'   `-' `-'   `-' `-'   `-' `-'   `-' `-'
> Skip Vaught-     jev at u.arizona.edu
> Brian Coullahan- coullaha at u.arizona.edu
> Mark Schwartz-   schwartm at u.arizona.edu
> Amy Raymond-     raymonda at u.arizona.edu


We have been using the Kloen for the last 9 months or so and found it to
be a very good product.  The learning curve was steep to begin
with.....lots of needles bent, broken, or clogged during the first two
weeks.  But after that we rarely need to replace a needle (<1 each
fortnight an average).  You have to be sure to rinse with di water
between each loading, but other than that, its very reliable.  

You might want to check out the new Robins system.  It is similar to the
Kloen, but it uses flexible tips instead of very thin metal tips.  We
have seen this device, but not tried it.  When Robins gets one to us so
we can test it, I will let people know on this newgroup how it works.

Hope that helps.

srl
-- 
Stephen R. Lasky, Ph.D.			#
University of Washington		#
Department of Molecular Biotechnology	#
srlasky at u.washington.edu		#
#########################################




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