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Taq sequencing

DNA Sequencing Facility dna-nbt at osu.edu
Fri May 14 17:14:24 EST 1999





Below is experimental evidence to support Philip San Miguel's response as
to the question about Taq fidelity.  In the lab for each seqeuncing gel, 75
so far, a sequencing reaction utilitzing the control plasmid and primer
from the ABI BigDye terminator kit was done and subsequently compared to
the published sequence  (from Promega Corp.) for  pGEM3zf.  For the first
400 bases of each reaction, which is the reliable read length of the gel,
there has never been a misincorporated base.  That is 16,000 bases without
a mistake for Taq polymerase.

Mike Zianni

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Michael Zianni
Neurobiotechnology  DNA Sequencing Facility
Ohio State University
038 Rightmire Hall
1060 Carmack Road
Columbus, OH  43210-1002

E-mail:  dna-nbt at osu.edu
Telephone:  614-292-3025
FAX:  614-292-5379
Web site: http://sfloor4.osbc.ohio-state.edu/neuro/DNA/home.html
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